Visible Spectrophotometric Methods for the Estimation of Losatan Potassium and Omeprazole in Single Component Pharmaceutical Formulations

Three simple new visible spectrophotometric methods has been developed for determination of Losartan potassium and Omeprazole, in single component pharmaceutical dosage forms. The methods were based on the formation of ion-pair complexes of the drug with dye Bromocresol purple(BCP) and Bromophenol blue(BPB), in acidic buffer solutions followed by their extraction in chloroform. Experiments were carried out to optimize the reaction conditions for complete colour formation. It was found that 5 ml BCP & BPB reagents and 5 ml of buffer solutions were optimum for the achievement of maximum colour intensity. BCP with Acid phthalate buffer of pH3 gave more absorbance at 430 nm this process was optimized(with BPB 400nm)The optical charactersic such as beer’s law, correlation coefficient, slope, Y intercept, and Molar absorptivity were calculated for all the methods and the analysis results of marketed formulations were in good agreement with their labeled claim. The recovery ranged from between 98 to 100 % for BCP& BPB which comply with official limits. The proposed methods were simple, sensitive, accurate and precise and can be successfully applied for the estimation of Losartan potassium and Omeprazole. Most of the Formulations contain excepients, which are added along with the active drug constituents. These substances may cause some interference during estimation of the active drug constituents, so the recovery study was employed. This was satisfactory & indicates the non-interferance of excipients. Results of the method were validated statistically and by recovery studies, this method was demonstrated to be adequate for routine analysis.